dynabeads oligo dt (Thermo Fisher)

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Thermo Fisher dynabeads oligo dt
Dynabeads Oligo Dt, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/dynabeads+oligo/Oligomycin/pm42019572-103-16-18
Average 97 stars, based on 1 article reviews

dynabeads oligo dt - by Bioz Stars, 2026-09

97/100 stars

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Purification:

Article Title: Regulatory patterns of testis and ovary maturation in largemouth bass (Micropterus salmoides) revealed by transcriptome profiling
Article Snippet: Largemouth bass (Micropterus salmoides) is an economically significant farmed fish.. It would be useful to understand its gametic maturation to establish effective breeding populations.. We conducted histological analyses, transcriptome sequencing, and fluorescent in situ hybridization (FISH) analyses to detect differences in gene expression between ovaries and testes of sexually mature largemouth bass (6.5 months old).

Article Title: MSC-encapsulated porous microparticle eye drops for autoimmune dry eye disease treatment in NOD mice
Article Snippet: RNA quantity and purity were assessed with NanoDrop ND-1000 (NanoDrop, USA), and integrity was confirmed using Bioanalyzer 2100 [Agilent, USA; RNA integrity number (RIN) > 7.0] and denaturing agarose gel electrophoresis. .. Poly(A) mRNA was isolated from 1 μg of total RNA using Dynabeads Oligo (dT)25-61005 (Thermo Fisher Scientific, USA) with two purification rounds, fragmented at 94°C for 5 to 7 min using the Magnesium RNA Fragmentation Module [New England Biolabs (NEB); catalog no. E6150, USA], and reverse-transcribed into cDNA with SuperScript II Reverse Transcriptase (Invitrogen, catalog no. 1896649, USA). .. Second-strand DNA was synthesized using Escherichia coli DNA polymerase I (NEB, catalog no. M0209, USA), ribonuclease H (NEB, catalog no. M0297, USA), and dUTP solution (Thermo Fisher Scientific, catalog no. R0133, USA).

Article Title: Intrauterine infusion of autologous platelet-rich plasma modulates endometrial immune status and improves pregnancy outcomes in patients with persistent chronic endometritis
Article Snippet: Total RNA was isolated using Trizol reagent according to the manufacturer’s procedure (Invitrogen, Carlsbad, CA, USA). .. Poly (A) RNA is purified from 1μg total RNA using Dynabeads Oligo (dT)25-61005 (Thermo Fisher, CA, USA) using two rounds of purification. .. Then the poly(A) RNA was fragmented into small pieces using a Magnesium RNA Fragmentation Module (NEB, cat. e6150, USA).

Article Title: OsLB2.2 negatively regulates rice disease resistance at seedling stage in rice
Article Snippet: The RNA integrity was assessed by Bioanalyzer 2100 (Agilent, CA, USA) with RIN number >7.0, and confirmed by electrophoresis with denaturing agarose gel. .. Poly (A) RNA is purified from 1μg total RNA using Dynabeads Oligo (dT)25-61005 (Thermo Fisher, CA, USA) using two rounds of purification. .. Then the poly(A) RNA was fragmented into small pieces using Magnesium RNA Fragmentation Module (NEB, cat.e6150, USA) under 94°C 5-7min.

Article Title: TRPC3 contributes to cyclophosphamide-induced cystitis progression by enhancing bladder fibrosis through activation of the TGF-β/smad pathway
Article Snippet: The RNA integrity was assessed using a Bioanalyzer 2,100 (Agilent, United States), with an RNA Integrity Number (RIN) greater than 7.0, and further confirmed by denaturing agarose gel electrophoresis. .. Poly(A) RNA was purified from 1 μg of total RNA using Dynabeads Oligo (dT)25-61005 (Thermo Fisher, United States) with two rounds of purification. .. The purified poly(A) RNA was then fragmented into small pieces using the Magnesium RNA Fragmentation Module (NEB, United States) at 94°C for 5–7 min.

Isolation:

Reverse Transcription:

Immunoprecipitation:

Article Title: Analysis of Non-Coding RNAs and N6-Methyladenosine-Modified Genes in Response to flg22 in Grape Immunity.
Article Snippet: Based on the predicted results of lncRNAs- miRNAs- mRNAs, the ceRNA networks diagram was drawn by Cytoscape (version 3.7.1). .. The mRNA enriched by Dynabeads Oligo (dT, Thermo Fisher, USA) was fragmented into short segments using Magnesium RNA Fragmentation Module (NEB, 6150, USA) and subjected to co- immunoprecipitated (IP) with an m6A- specific antibody (Synaptic Systems). ..

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